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Once acetaldehyde is obtained from ethanol metabolism, aldehyde dehydrogenase (ALDH) converts it into acetate, but if the system is overloaded, the excess of acetaldehyde reacts with mitochondrial GSH (mGSH), depleting the mitochondrial pool [143]

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Composed of the amino acids L-cysteine, L-glutamic acid, and glycine, the tripeptide is synthesized by the addition of the C-terminal of gamma-glutamylcysteine catalyzed by glutathione synthetase