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For HeLa cells, permeabilization was performed using 0.5% Triton X-100 in PBS, followed by incubation with anti-RIPK1(1:50, BD Biosciences, 610459) and anti-STK38 (1:200, Proteintech, 55335-1-AP) antibodies diluted in 2.5% BSA in PBS at RT overnight

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Lasers used in this procedure include erbium and thulium fiber lasers