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Determination of intraparasitic response to oxidative stress levels by measuring the oxidation of 2′, 7′-dichlorodihydrofluorescein (DCFH) To determine the levels of intraparasitic response to oxidative stress , the fluorescent intensity of dichlorofluorescein (DCF), the green fluorescent oxidation product of DFCH, was determined in WT and pbggcs-ko parasites cultured in vitro