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Cellular viability Optimization of Sp concentration and its cytotoxicity parameters were established by treating of 1.5 × 10 5 HeLa or 1.2 × 10 5 SHSY-5Y cells with serial concentrations of the Sp (100 µM–1 mM) for 15 min or conducting a time-course (5 min–48 h) using 250 µM Sp, followed by MTT assay as per standard protocol (Thermo Fisher Scientific)
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