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Following a washing step, Alexa Fluor 594-conjugated goat anti-rabbit IgG (1:400, Molecular Probes, Waltham, MA, USA) and/or Alexa Fluor 488-conjugated goat anti-mouse IgG (1:400, Molecular Probes) were/was added as the secondary antibody and DAPI was used for nuclear staining
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It was demonstrated that the recovery is due to BPC 157-activated azygos vein direct blood flow delivery serving as an upgraded minor vessel that may quickly act for the failed (compressed) major vessel, reestablishing the reorganized blood flow [18,19,20,21,22,23,24,25,26,27,28,29,30]
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